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  • CircRNA CRDMO

  • Product Introduction
  • Case Analysis


  • circRNA CRDMO Service


    Circular RNA, with greater stability attributed to its unique closed-loop structure, is an ideal platform for the development of next-generation gene therapy as an effective alternative to linear mRNA. Geneseed has been focusing on circRNA for more than 10 years and has profound technology accumulation. Geneseed has two patented RNA circularization strategies: Group Ⅰintron self-splicing and T4 Rnl2 method. We have established platforms for sequence design, process development, analytical method development, scale production, QC, raw material preparation, etc., which can provide biopharma with CRDMO services in the whole process from early R&D to commercial production.



    Our Serveice





    Manufacturing Process from Plasmid to circRNA





    Quality Analysis of circRNA





    Quality Analysis of circRNA-LNP





    Advantages

    1. Two independent intellectual property rights, comprehensively matching the needs of the project：circPrecise? by Group I intron self-splicing，circPure? by T4 Rnl2；

    2. Unique sequence design approach，circularization efficiency＞90%：using modular translation element screening and secondary structure prediction of IRES and CDS interaction, the translation efficiency is greatly improved；

    3. Robust amplification production process，mass production scale up：flexible IVT- circularization scale from μg to g grade for different templates；

    4. Various purification strategies，purity＞95%：analyze impurity components and concentration to customize a personalized chromatographic purification strategy；

    5. Common LNP encapsulation platform，efficiency＞95%：self-developed liposome screening, platform for different formulations；

    6. Multi-dimensional QC platform：develop analytical methods to match the needs of project, and meet the QC evaluation of each stage;

    7. Efficient raw material synthesis platform to reduce production costs significantly.





    Catalog CircRNA Products


    Except for circ-EPO, circ-Trp53, circ-TP53, circ-eGFP, circ-Fluc, circ-mCherry and Natural Circular RNA, 3xFlag labels were inserted to other Catalog CircRNA Products, and anti-Flag antibodies can be used to detect their protein products.

    Product    Category

    Name

    Model

    Specification

    Reporter gene

    GSPure? Circ-eGFP

    R0701 / R0702 / R0703 / R0704

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-Fluc

    R0801 / R0802 / R0803 / R0804

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-mCherry

    R0901 / R0902 / R0903 / R0904

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-beta gal

    R1401 / R1402 / R1403 / R1404

    100 μg / 500 μg / 1 mg / 5 mg

    Tumor antigen

    GSPure? Circ-OVA

    R1501 / R1502 / R1503 / R1504

    100 μg / 500 μg / 1 mg / 5 mg

    Anti-tumor

    GSPure? Circ-TP53

    R1701 / R1702 / R1703 / R1704

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-Trp53

    R2501 / R2502 / R2503 / R2504

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-PTEN-L (homo)

    R1001 / R1002 / R1003 / R1004

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-PTEN-L (mmu)

    R1101 / R1102 / R1103 / R1104

    100 μg / 500 μg / 1 mg / 5 mg

    Growth factor

    GSPure? Circ-VEGFA165 (homo)

    R1201 / R1202 / R1203 / R1204

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-VEGFA165 (mmu)

    R1301 / R1302 / R1303 / R1304

    100 μg / 500 μg / 1 mg / 5 mg

    immunotherapy

    GSPure? Circ-IL2 (homo)

    R1901 / R1902 / R1903 / R1904

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-IL2 (mmu)

    R3201 / R3202 / R3203 / R3204

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-IL12a

    R3301 / R3302 / R3303 / R3304

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-IL12A

    R3401 / R3402 / R3403 / R3404

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-IL12B

    R3501 / R3502 / R3503 / R3504

    100 μg / 500 μg / 1 mg / 5 mg

    Gene editing

    GSPure? Circ-Cas9

    R2001 / R2002 / R2003 / R2004

    100 μg / 500 μg / 1 mg / 5 mg

    Protein/

    Antibody replacement

    GSPure? Circ-EPO (homo)

    R2101 / R2102 / R2103 / R2104

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-EPO (mmu)

    R2301 / R2302 / R2303 / R2304

    100 μg / 500 μg / 1 mg / 5 mg

    iPSC reprogramming

    GSPure? Circ-SOX2 (homo)

    R2201 / R2202 / R2203 / R2204

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-SOX2 (mmu)

    R2401 / R2402 / R2403 / R2404

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-OCT4 (homo)

    R2601 / R2602 / R2603 / R2604

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-OCT4 (mmu)

    R2701 / R2702 / R2703 / R2704

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-KLF4 (homo)

    R2801 / R2802 / R2803 / R2804

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-KLF4 (mmu)

    R2901 / R2902 / R2903 / R2904

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-C-Myc (homo)

    R3001 / R3002 / R3003 / R3004

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-C-Myc (mmu)

    R3101 / R3102 / R3103 / R3104

    100 μg / 500 μg / 1 mg / 5 mg

    Anti-aging

    GSPure? Circ-TERT

    R3601 / R3602 / R3603 / R3604

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-Tert

    R3701 / R3702 / R3703 / R3704

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-FGF21 (homo)

    R3801 / R3802 / R3803 / R3804

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-FGF21 (mmu)

    R3901 / R3902 / R3903 / R3904

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-αKlotho (homo)

    R4001 / R4002 / R4003 / R4004

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-αKlotho (mmu)

    R4101 / R4102 / R4103 / R4104

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-sTGFβR2 (homo)

    R4201 / R4202 / R4203 / R4204

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-sTGFβR2 (mmu)

    R4301 / R4302 / R4303 / R4304

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-FGF23 (homo)

    R4401 / R4402 / R4403 / R4404

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-FGF23 (mmu)

    R4501 / R4502 / R4503 / R4504

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-FGF17 (homo)

    R4601 / R4602 / R4603 / R4604

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-FGF17 (mmu)

    R4701 / R4702 / R4703 / R4704

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-SOX8 (homo)

    R4801 / R4802 / R4803 / R4804

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-SOX8 (mmu)

    R4901 / R4902 / R4903 / R4904

    100 μg / 500 μg / 1 mg / 5 mg

    Neurotrophic factor

    GSPure? Circ-NGF (homo)

    R5001 / R5002 / R5003 / R5004

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-NGF (mmu)

    R5101 / R5102 / R5103 / R5104

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-BDNF (homo)

    R5201 / R5202 / R5203 / R5204

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-BDNF (mmu)

    R5301 / R5302 / R5303 / R5304

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-GDNF (homo)

    R5401 / R5402 / R5403 / R5404

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-GDNF (mmu)

    R5501 / R5502 / R5503 / R5504

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-NT3 (homo)

    R5601 / R5602 / R5603 / R5604

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-NT3 (mmu)

    R5701 / R5702 / R5703 / R5704

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-NT4 (homo)

    R5801 / R5802 / R5803 / R5804

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-NT4 (mmu)

    R5901 / R5902 / R5903 / R5904

    100 μg / 500 μg / 1 mg / 5 mg

    Nerve regeneration

    transforming factor

    GSPure? Circ-NeuroD1 (homo)

    R6001 / R6002 / R6003 / R6004

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-NeuroD1 (mmu)

    R6101 / R6102 / R6103 / R6104

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-NeuroD2 (homo)

    R6201 / R6202 / R6203 / R6204

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-NeuroD2 (mmu)

    R6301 / R6302 / R6303 / R6304

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-NeuroD6 (homo)

    R6401 / R6402 / R6403 / R6404

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-NeuroD6 (mmu)

    R6501 / R6502 / R6503 / R6504

    100 μg / 500 μg / 1 mg / 5 mg

    Natural circular RNA

    GSPure? Circ-PVT1 (homo)

    R6601 / R6602 / R6603 / R6604

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-PVT1 (mmu)

    R6701 / R6702 / R6703 / R6704

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-FOXO3 (homo)

    R6801 / R6802 / R6803 / R6804

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-FOXO3 (mmu)

    R6901 / R6902 / R6903 / R6904

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-HIPK3

    R7001 / R7002 / R7003 / R7004

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-ZNF609

    R7101 / R7102 / R7103 / R7104

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-ZFR

    R7201 / R7202 / R7203 / R7204

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-SMARCA5

    R7301 / R7302 / R7303 / R7304

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-PRKCI

    R7401 / R7402 / R7403 / R7404

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-NRIP1

    R7501 / R7502 / R7503 / R7504

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-ABCB10

    R7601 / R7602 / R7603 / R7604

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-CDYL

    R7701 / R7702 / R7703 / R7704

    100 μg / 500 μg / 1 mg / 5 mg

    GSPure? Circ-Mbl

    R7801 / R7802 / R7803 / R7804

    100 μg / 500 μg / 1 mg / 5 mg



  • High integrity


    Figure 1: GSPure? Circ-eGFP (left) and GSPure? Circ-mCherry (right) integrity (CE) > 95% after purification




    High purity


    Figure 2: GSPure? Circ-eGFP (left) and GSPure? Circ-Fluc (right) purity (HPLC) > 95% after purification



    High quality


    Fig 3: Fluorescence detection results after GSPure?Circ-eGFP transfected 293T cells, magnification: 100×. 






    Fig 4: Fluorescence detection results after GSPure? Circ-mCherry transfected 293T cells, magnification: 100×. 




    Fig 5: Results of luciferase detection after GSPure? Circ-Fluc transfection of 293T cells.